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Accelerated Discovery Nanobody Platformbroad

ALADDIN · Horizon Europe grant · 2024-01-01–2027-12-31

EC contribution

€3,315,441

Total cost

€3,315,441

Beneficiaries

8
About the data

Source: CORDIS (official EU open data), Horizon Europe. Framework HORIZON · call HORIZON-EIC-2023-PATHFINDEROPEN-01 · scheme HORIZON-EIC · topic HORIZON-EIC-2023-PATHFINDEROPEN-01-01. CORDIS record →

Objective

Since the approval of the first monoclonal antibody (mAb) 30 years ago, therapeutic mAbs and Ab-derived molecules have come to dominate the biologics market. Currently, +100 Abs are in clinical use for different diseases, out of which +30 mAb target cancer. Despite the huge market potential, multiple and complex steps make the therapeutic Ab discovery process long, expensive, laborious, and inefficient. These steps include target identification, animal immunization, Ab selection and engineering, humanization and preclinical validation. All these are not integrated, costly, require large equipments and facilities, and are highly dependent on experimental animals (mostly mammals, including genetically modified mice), both for immunization and for preclinical validation of the therapeutic Ab candidates.ALADDIN project emerges to bring to the market a novel AcceLerAteD DIscovery Nanobody platform that will increase the efficiency of therapeutic Ab discovery and preclinical validation for human cancer by: 1) integrating selection and in vivo affinity maturation of Abs in bacterial cells holding an universal library of single domain Abs (nanobodies, Nbs) that fully eliminates animal inmunization; 2) using in silico Artificial Intelligent (AI) tools for structure-based epitope mapping, AI-guided affinity maturation, and Nb humanization; 3) developing cost-effective miniaturized microfluidic-based devices for in vitro Ab selection from bacterial cultures; 4) accelerating Ab validation with a fast non-mammalian in vivo model for preclinical testing based on patient-derived tumor xenografts in zebrafish larvae; 5) impacting target and Ab validation with dynamic mathematical models to extract clinical and efficacy data of the Ab candidates. These ambitious goals will be possible through the multidisciplinary ALADDIN consortium, formed by eight partners with complementary skills.

Beneficiaries (8)

OrganisationCountryRoleEC contributionSME
AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS ES coordinator €595,062
LINKOPINGS UNIVERSITET SE participant €506,344
INTERNATIONAL IBERIAN NANOTECHNOLOGY LABORATORY PT participant €426,722
INCELLIA IKE EL participant €376,522 Yes
INSTITUTO DE SALUD CARLOS III ES participant €374,745
THE HEBREW UNIVERSITY OF JERUSALEM IL participant €374,350
INSTITUTO INVESTIGACION SANITARIA FUNDACION JIMENEZ DIAZ ES participant €362,750
ZIRKA INNOTECH SL ES participant €298,945 Yes

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