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Hydrogen oxidizing bacteria engineered to valorize CO2 for whey protein productionbroad

HYDROCOW · Horizon Europe grant · 2023-09-01–2028-02-29

EC contribution

€3,963,836

Total cost

€3,963,836

Beneficiaries

6
About the data

Source: CORDIS (official EU open data), Horizon Europe. Framework HORIZON · call HORIZON-EIC-2022-PATHFINDERCHALLENGES-01 · scheme HORIZON-EIC · topic HORIZON-EIC-2022-PATHFINDERCHALLENGES-01-01. CORDIS record →

Objective

Milk protein plays an important role in our nutrition, however classical milk production has significant environmental impacts, from greenhouse gas (GHG) emissions to extensive land demand. Project HYDROCOW addresses these challenges through a net-zero carbon dairy protein production platform. The main objective of the project isto develop and demonstrate a bacterial protein secretion system where CO2 and soon N2 is valorised into food-grade protein, decoupled from agriculture. This system will base on the first-of-a-kind engineered hydrogen oxidizing bacterium (eHOB) Xanthobacter sp. SoF1. As a first product the main milk component beta-lactoglobulin was chosen.Technically this will be achieved by implementing a Design-Build-Test-Learn (DBTL) cycle linked to a validation and scale-up phase allowing to iteratively optimize the production of secreted protein. The project will deliver key technologies – A) an innovative eHOB protein secretion system; B) predictive eHOB metabolic models, genetic engineering tools, and a novel high-throughput (HTP) screening system for DBTL cycling; and C) the methods for validation and scale-up – with immediate and long-term impact on the production of food and nutrition, materials, medicines, fuels and chemicals. In the long-term, the proposed platform has the potential to not only replace conventionally produced food proteins but also deliver proteins for materials or therapeutics, important for human and animal health. In comparison to current standard microbial production processes our platform does not compete with human nutrition for valuable feedstock, such as glucose, and therefore will contribute to a sustainable development of our society. HYDROCOW will generate significant knowledge for a growing research and application community about autotrophic, microbial production systems, their physiology, and sophisticated tools for genetically designing and screening them.

Beneficiaries (6)

OrganisationCountryRoleEC contributionSME
SOLAR FOODS OYJ FI coordinator €1,626,325 Yes
RIJKSUNIVERSITEIT GRONINGEN NL participant €1,430,351
RHEINISCH-WESTFAELISCHE TECHNISCHE HOCHSCHULE AACHEN DE participant €907,160
IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE UK associatedPartner
FGEN AG CH associatedPartner Yes
EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH CH associatedPartner

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