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Hacking the ribosome to map virus-host associationsbroad

VirHoX · Horizon Europe grant · 2025-02-01–2029-01-31

EC contribution

€3,560,000

Total cost

€3,560,000

Beneficiaries

9
About the data

Source: CORDIS (official EU open data), Horizon Europe. Framework HORIZON · call HORIZON-EIC-2024-PATHFINDEROPEN-01 · scheme HORIZON-EIC · topic HORIZON-EIC-2024-PATHFINDEROPEN-01-01. CORDIS record →

Objective

High throughput sequencing and metagenomics have deepened our understanding of viruses in nature, yet linking viruses to their hosts remains a challenge, hindering our knowledge and applications. The VirHoX project aims to overcome this by ""hacking"" the ribosome to map virus-host associations. Leveraging a critical moment in the infection cycle of all viruses, the translation of viral genes into proteins by the host cell, we will uncover concealed relationships between viruses and their hosts, providing a universal tool for virus-host pairing. This novel approach, VirHo-seq, involves ligating host ribosomal RNA with viral mRNA during translation in infected cells using engineered enzymes, followed by high throughput sequencing to reveal virus-host pairings. Our interdisciplinary consortium of academic and industrial experts in microbiology, protein engineering, gene editing, viral genomics/transcriptomics and law, will develop and then prepare the commercialisation of VirHo-seq, envisioning legislative and analytical frameworks to maximise its impact. This technology will significantly help tackle current challenges, such as those posed by emerging pathogens, ecological disruptions, and climate change. Our approach aligns with the EIC Work Programme objectives, demonstrating feasibility on model organisms before validating on relevant samples, addressing the ""engineered living materials"" challenge.""

Beneficiaries (9)

OrganisationCountryRoleEC contributionSME
UNIVERSITA DEGLI STUDI DI PADOVA IT coordinator €796,000
MIEDZYNARODOWY INSTYTUT BIOLOGII MOLEKULARNEJ I KOMORKOWEJ W WARSZAWIE PL participant €504,000
UNIVERSITETET I OSLO NO participant €441,468
INSTITUT PASTEUR FR participant €437,276
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS FR participant €394,918
INSTITUTE OF SCIENCE AND TECHNOLOGY AUSTRIA AT participant €375,315
UNIVERSITA DEGLI STUDI DI TRIESTE IT participant €333,299
FIZ KARLSRUHE - LEIBNIZ-INSTITUT FUR INFORMATIONSINFRASTRUKTUR GMBH DE participant €227,725
Eubiome Srl IT participant €50,000 Yes

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