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Microglia As conTroller of braIn metaboLism During Agingcore

MATILDA · Horizon Europe grant · 2023-01-01–2028-12-31

EC contribution

€2,500,000

Total cost

€2,500,000

Beneficiaries

1
About the data

Source: CORDIS (official EU open data), Horizon Europe. Framework HORIZON · call ERC-2021-ADG · scheme HORIZON-ERC · topic ERC-2021-ADG. CORDIS record →

Objective

Microglia represent the main brain residential immune cells. In the last years evidence emerged that, besides representing the first line of defense against pathogenic insults, microglia are also centrally involved in physiological functions essential for correct CNS development and function. Microglia, in a spatially and temporally controlled manner, influence neuronal apoptosis, neurogenesis and myelin formation and manage synapse homeostasis, including removing supernumerary synapses during development. These widely heterogeneous roles are supported by distinct subtypes -or states- of microglia, present in different regions of the brain and at different times during CNS development. In the last years, Triggering Receptor Expressed on Myeloid cells 2 (Trem2) emerged as a key gene which controls the microglial metabolic state, in addition to directing synapse elimination and shaping the functional brain connectivity. In the present project, I propose that microglia, via Trem2, direct the genetic signature of specific subgroups of brain cells, shifting them toward specific metabolic and developmental patterns. I hypothesize that this mechanism is key for the control of metabolism in the hypothalamus, where the neuronal contingents orchestrating systemic energy homeostasis reside. By using a combination of transcriptomics, multiplex protein expression analysis and cell-based imaging methods, I will determine which subsets of microglia cooperate within hypothalamic centers and will define the underlying mechanisms. I will also assess whether defects in this orchestrated crosstalk affect the central regulation of energy and glucose homeostasis during aging. Data from this study will provide comprehensive knowledge of microglia functions in shaping hypothalamic complexity and endocrine output. Also, they will offer a targeting potential for new therapeutic strategies that could reverse immunometabolic dysfunction by modulation of hypothalamic microglial function.

Beneficiaries (1)

OrganisationCountryRoleEC contributionSME
HUMANITAS MIRASOLE SPA IT coordinator €2,500,000

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