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Development of an In-Vivo Brillouin Microscope (with application to Protein Aggregation-based Pathologies)broad

IVBM-4PAP · Horizon Europe grant · 2023-03-01–2027-08-31

EC contribution

€3,868,938

Total cost

€3,868,938

Beneficiaries

6
About the data

Source: CORDIS (official EU open data), Horizon Europe. Framework HORIZON · call HORIZON-EIC-2022-PATHFINDEROPEN-01 · scheme HORIZON-EIC · topic HORIZON-EIC-2022-PATHFINDEROPEN-01-01. CORDIS record →

Objective

The role and importance of mechanical properties of cells and tissues in cellular function, development and disease has widely beenacknowledged, however standard techniques currently used to assess them exhibit intrinsic limitations (invasive, lack of 3D capabilityand of sub-cellular resolution). Recently, Brillouin Microscopy (BM), a type of optical elastography, has emerged as a non-destructive,label- and contact-free method that can probe the viscoelastic properties of biological samples with diffraction-limited resolution in3D. This led to increased attention amongst the biological and medical research communities. However, due to the long acquisitiontime (hours), this novel technique has been applied only to fixed samples. One important open challenge is to use this approach tofollow the mechano-biological processes in living cells and in real time. Aim of our project is overcome the spectral-imagingacquisacknowledged, however standard techniques currently used to assess them exhibit intrinsic limitations (invasive, lack of 3D capability and of sub-cellular resolution). Recently, Brillouin Microscopy (BM), a type of optical elastography, has emerged as a non-destructive, label- and contact-free method that can probe the viscoelastic properties of biological samples with diffraction-limited resolution in 3D. This led to increased attention amongst the biological and medical research communities. However, due to the long acquisition time (hours), this novel technique has been applied only to fixed samples. One important open challenge is to use this approach to follow the mechano-biological processes in living cells and in real time. Aim of our project is overcome the spectral-imaging acquisition time limitation by applying the heterodyne detection scheme, allowing the collection of viscoelastic properties in living sample at a sub-second timescale. We will attempt solving specific physio-pathological open problems in biomedicine for the first time.

Beneficiaries (6)

OrganisationCountryRoleEC contributionSME
FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA IT coordinator €1,627,508
UNIVERSITA DEGLI STUDI DI TRENTO IT participant €534,610
UNIVERSIDAD DE ZARAGOZA ES participant €530,199
UNIVERSIDADE DE COIMBRA PT participant €499,790
CRESTOPTICS S.P.A. IT participant €497,581
UNIVERSITE D'ANGERS FR participant €179,250

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